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Therefore, this data set is unique, to our knowledge, and allowed a comprehensive analysis of rhythmic genes, protein expression, and associated metabolic pathways, as well as the effect of the deletion of the circadian clock regulator BMAL1 on these processes (of note, BMAL1 is a transcription factor that may have functions unrelated to the circadian clock)

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Two acidic residues and no basic residue leave the molecule anionic at physiological pH, and a negatively charged mucosal surface works against passage of an anion