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At 48 h after plating, the medium was replaced with DMEM supplemented with 5% horse serum (Sigma), 20 mM D-glucose and, on day 4, cytosine arabinoside (10 μM) to prevent non-neuronal proliferation 33

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In laboratory studies, BPC-157 has been observed to modulate NO synthase activity, potentially influencing blood vessel formation and endothelial cell behavior

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